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          基因商城首頁 > 試劑 > 測序 > 文庫構建 > DNA建庫

          NEBNext DirectTM Cancer HotSpot Panel

          NEBNext Direct?Cancer HotSpot Panel

          品牌:NEB 貨號:E7000S 注冊及訂單:注冊及訂單 咨詢QQ 產品及技術:產品及技術 咨詢QQ

          商城價:

          ¥11423.00
          目錄價:¥13439.00

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          ¥11423.00
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          • 基因生物技術國際貿易(上海)有限公司 發貨并提供售后服務。
            本產品僅限科研用途。

          產地:USA
          存儲條件:2-8 & -20
          靶向富集,配合 NGS 技術,使對基因組中目標區域高通量、高深度的測序成為可能。 NEBNext Direct 是一種全新的,基于雜交的捕獲方法,與傳統的液相雜交及多重 PCR 方法相比有明顯的優勢。 在 NEBNext Direct 靶向富集方法中(圖1),片段化的 DNA 迅速雜交到生物素化的寡聚核苷酸餌上,該寡聚核苷酸定義了目標序列 3’ 端序列。核苷酸餌-目標序列雜交體結合到鏈霉親和素磁珠上,而任何 3’ 脫靶序列則通過酶學法去除。短雜交時間搭配酶學法去除 3’ 脫靶序列的方法與傳統的基于雜交的富集方法相比,有著更高的測序效率。這些經過修飾的目標序列隨后被轉化為含有分子標簽(UMI)及樣本 barcode 的文庫,與 Illumina 平臺兼容。可用于上機測序的文庫可以在一天之內制備完成。整個制備流程與大多數自動化儀器設備兼容。 NEBNext Direct HotSpot Cancer Panel 中的寡聚核苷酸餌能夠捕獲 50 個基因中 190 個常見癌癥目標序列的雙鏈 DNA,能夠涵蓋 40 kb 的序列,包括18,000 個COSMIC 數據庫中所述情況。本 panel 適用于構建約 150 bp 的文庫,適用于Illumina 平臺 PE75 測序。 優勢: 可比對到目標區域的reads 比例更高; 避免過度測序,減少每個樣本的花費; 所有區域均可得到均一的測序結果,無論 GC 含量多少; 將富集和文庫制備流程相結合,1 天即可完成; 能夠從有限及降解的 DNA 樣品中得到高質量的文庫,包括 FFPE 及 ctDNA; 區分分子重復,降低假陽性突變并提高敏感度;該試劑盒的質控是獨立管理的并通過功能驗證。每個組分均通過標準的酶活性檢測及質控,并通過嚴格的額外質量控制,質控情況可在每個組分的單獨頁面查詢。
          Target enrichment, coupled with next generation sequencing (NGS), enables high throughput, deep sequencing of genomic regions of interest. NEBNext Direct is a novel, hybridization-based capture method offering significant advantages over traditional in-solution hybridization and multiplex PCR protocols. In the NEBNext Direct target enrichment approach (Figure 1), fragmented DNA is rapidly hybridized to biotinylated oligonucleotide baits that define the 3′ end of each target of interest. The bait-target hybrids are bound to streptavidin beads and any 3′ off target sequence is removed enzymatically. This combination of a short hybridization time with the enzymatic removal of 3′ off target sequence enables greater sequencing efficiency relative to conventional hybridization-based enrichment methods. The trimmed targets are then converted into Illumina-compatible libraries that include unique molecular identifiers (UMI) and a sample barcode. Sequence-ready libraries are generated within one day. The procedure is compatible with most automated liquid handling instruments. The NEBNext Direct HotSpot Cancer Panel contains baits that capture both strands of DNA across 190 common cancer targets from 50 genes, encompassing approximately 40 kb of sequence and including over 18,000 COSMIC features (Table 1). The panel is designed to generate targets of roughly 150 bp, compatible with PE75 Illumina sequencing. Advantages Generate a higher percentage of your sequencing reads aligning to your targets Eliminate the need to over-sequence, reducing cost per sample Obtain uniform sequencing of all targets, regardless of GC content Save time with a 1-day workflow that combines enrichment with library preparation Generate high quality libraries with limited input amounts and degraded DNA samples, including FFPE and ctDNA Distinguish molecular duplicates, reducing false positive variants and improving sensitivity
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